Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest

Curr Protoc Immunol. 2016 Nov 1:115:7.21C.1-7.21C.12. doi: 10.1002/cpim.15.

Abstract

Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described. © 2016 by John Wiley & Sons, Inc.

Keywords: HVS-transformed T cell; T cells; herpesvirus saimiri-transformed T cells; retrovirus; transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Transformed
  • Gene Order
  • Gene Transfer Techniques
  • Genetic Vectors / genetics*
  • Herpesvirus 2, Saimiriine / genetics*
  • Humans
  • Plasmids / genetics
  • Retroviridae / genetics
  • T-Lymphocytes / metabolism*
  • Transduction, Genetic / methods*
  • Transgenes*