Backbone resonance assignments of the F-actin binding domain of mouse αN-catenin

Tadateru Nishikawa; Noboru Ishiyama; Feng Wang; Mitsuhiko Ikura

doi:10.1007/s12104-016-9713-8

Backbone resonance assignments of the F-actin binding domain of mouse αN-catenin

Biomol NMR Assign. 2017 Apr;11(1):21-24. doi: 10.1007/s12104-016-9713-8. Epub 2016 Nov 1.

Authors

Tadateru Nishikawa¹, Noboru Ishiyama¹, Feng Wang^{1

2}, Mitsuhiko Ikura³

Affiliations

¹ Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, M5G 1L7, Canada.
² Department of Biochemistry, School of Medicine, Vanderbilt University, 2215 Garland Ave., 607 Light Hall, Nashville, TN, 37232-0146, USA.
³ Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, M5G 1L7, Canada. [email protected].

PMID: 27804064
DOI: 10.1007/s12104-016-9713-8

Abstract

α-Catenin is a filamentous actin (F-actin) binding protein that links the classical cadherin-catenin complex to the actin cytoskeleton at adherens junctions (AJs). Its C-terminal F-actin binding domain is required for regulating the dynamic interaction between AJs and the actin cytoskeleton during tissue development. Thus, obtaining the molecular details of this interaction is a crucial step towards understanding how α-catenin plays critical roles in biological processes, such as morphogenesis, cell polarity, wound healing and tissue maintenance. Here we report the backbone atom (¹H^N, ¹⁵N, ¹³C^α, ¹³C^β and ¹³C') resonance assignments of the C-terminal F-actin binding domain of αN-catenin.

Keywords: Cell junction; F-actin; Mechanosensor; α-Catenin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism*
Animals
Mice
Nuclear Magnetic Resonance, Biomolecular*
Protein Domains
alpha Catenin / chemistry*
alpha Catenin / metabolism*

Substances

Actins
alpha Catenin

Grants and funding

MOP 130267/CIHR/Canada