Digitization of Endocytic pH by Hybrid Ultra-pH-Sensitive Nanoprobes at Single-Organelle Resolution

Yiguang Wang; Chensu Wang; Yang Li; Gang Huang; Tian Zhao; Xinpeng Ma; Zhaohui Wang; Baran D Sumer; Michael A White; Jinming Gao

doi:10.1002/adma.201603794

Digitization of Endocytic pH by Hybrid Ultra-pH-Sensitive Nanoprobes at Single-Organelle Resolution

Adv Mater. 2017 Jan;29(1):10.1002/adma.201603794. doi: 10.1002/adma.201603794. Epub 2016 Nov 4.

Authors

Yiguang Wang^{1

2}, Chensu Wang^{1

3}, Yang Li¹, Gang Huang¹, Tian Zhao¹, Xinpeng Ma¹, Zhaohui Wang¹, Baran D Sumer⁴, Michael A White³, Jinming Gao¹

Affiliations

¹ Department of Pharmacology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
² Beijing Key Laboratory of Molecular Pharmaceutics and State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100191, China.
³ Department of Cell Biology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.
⁴ Department of Otolaryngology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX, 75390, USA.

Abstract

A multispectral hybrid nanotransistor consisting of modular fluorescent block copolymers with discrete and sharp pH transitions in one nanoparticluate system is presented. This nanotransistor probe allows digitized reporting of dynamic maturation at single-organelle resolution over time. This nanotechnology platform offers a powerful tool in fundamental studies of organelle biology, cell signaling in diseases characterized by pH dysregulation in the endosomes or lysosomes.

Keywords: acidification; endocytosis; hybrid nanoprobes; organelle maturation; pH-sensitive nanoparticles.

Abstract

Grants and funding