Sprayable peptide-modified silver nanoparticles as a barrier against bacterial colonization

Nanoscale. 2016 Nov 24;8(46):19200-19203. doi: 10.1039/c6nr07976h.

Abstract

Sprayable formulations of AgNPs were prepared by exchanging citrate capping agents with LL37-SH peptides. The AgNP@LL37 material was then combined with type I collagen to form a stable film once sprayed. The AgNP@LL37 spray prevented Pseudomonas aeruginosa (P. aeruginosa) proliferation and eradicated a P. aeruginosa biofilm, while being non-toxic for human skin fibroblasts embedded within 3D artificial skin constructs. Finally, no silver infiltration was observed after spraying on a full-thickness skin wound in a mouse model.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Cells, Cultured
  • Fibroblasts / microbiology
  • Humans
  • Metal Nanoparticles*
  • Peptides
  • Pseudomonas aeruginosa / drug effects
  • Silver / pharmacology*
  • Wounds and Injuries / drug therapy*

Substances

  • Anti-Bacterial Agents
  • Peptides
  • Silver