Inhibition of the CD3-mediated calcium signal by protein kinase C activators in human T (Jurkat) lymphoblastoid cells

Immunol Lett. 1989 Jan 15;20(1):47-52. doi: 10.1016/0165-2478(89)90067-9.

Abstract

The changes in the cytoplasmic free calcium concentrations (Cai) were investigated in human T (Jurkat) lymphoblastoid cells, loaded with the calcium-sensitive fluorescent dye Indo-1. A rapid increase in Cai could be evoked by monoclonal antibodies (mAb) directed against the CD3 antigen, as well as by the mitogenic lectin concanavalin A (conA). The protein kinase C (pKC) activators TPA and DiC8 did not increase Cai, but the addition of any of these two compounds prior to mAb eliminated the mAb-induced calcium signal. In contrast, the calcium signal evoked by ConA was not blocked by TPA or DiC8. These results suggest that the CD3-dependent calcium signal is selectively down-regulated by the activation of pKC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / physiology*
  • Calcium / pharmacology*
  • Cell Line
  • Depression, Chemical
  • Diglycerides / pharmacology*
  • Enzyme Activation / drug effects
  • Glycerides / pharmacology*
  • Humans
  • Lymphocyte Activation / drug effects*
  • Phorbol 12,13-Dibutyrate / pharmacology*
  • Protein Kinase C / physiology*
  • Receptors, Antigen, T-Cell / analysis
  • Signal Transduction / drug effects*
  • T-Lymphocytes / drug effects*
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Tumor Cells, Cultured / drug effects

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Diglycerides
  • Glycerides
  • Receptors, Antigen, T-Cell
  • 1,2-dioctanoylglycerol
  • Phorbol 12,13-Dibutyrate
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate
  • Calcium