Prevalence of Colistin Resistance Gene mcr-1 and Absence of mcr-2 in Escherichia coli Isolated from Healthy Food-Producing Animals in Japan

Michiko Kawanishi; Hitoshi Abo; Manao Ozawa; Mariko Uchiyama; Takahiro Shirakawa; Satowa Suzuki; Ayaka Shima; Akifumi Yamashita; Tsuyoshi Sekizuka; Kengo Kato; Makoto Kuroda; Ryoji Koike; Mayumi Kijima

doi:10.1128/AAC.02057-16

Prevalence of Colistin Resistance Gene mcr-1 and Absence of mcr-2 in Escherichia coli Isolated from Healthy Food-Producing Animals in Japan

Antimicrob Agents Chemother. 2016 Dec 27;61(1):e02057-16. doi: 10.1128/AAC.02057-16. Print 2017 Jan.

Authors

Affiliations

¹ National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan [email protected].
² National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Japan.
³ Department of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
⁴ Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Japan.

Abstract

We screened mcr-1 and mcr-2 genes in 9,306 Escherichia coli strains isolated from healthy animals in the Japanese Veterinary Antimicrobial Resistance Monitoring (JVARM) system. mcr-1 was detected in 39 strains (5, 20, and 14 strains isolated from cattle, swine, and broilers, respectively), whereas mcr-2 was not detected. mcr-2 was also not detected with the investigation sequence homology search against our curated GenEpid-J database.

Keywords: GenEpid-J; Japanese Veterinary Antimicrobial Resistance Monitoring system; colistin resistance; food-producing animals; mcr-1; mcr-2.

MeSH terms

Animal Husbandry
Animals
Anti-Bacterial Agents / pharmacology
Cattle
Chickens
Colistin / pharmacology
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / growth & development
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Expression
Japan / epidemiology
Meat / microbiology*
Poultry Diseases / epidemiology*
Poultry Diseases / microbiology
Prevalence
Protein Isoforms / genetics
Protein Isoforms / metabolism
Swine
Swine Diseases / epidemiology*
Swine Diseases / microbiology

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-1 protein, E coli
Protein Isoforms
Colistin