Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator)

Steven Van Borm; Laís S Rizotto; Leila S Ullmann; Guilherme P Scagion; Camila D Malossi; Raphael M Simão; João P Araújo Jr; Izabelle M Cordeiro; Lara B Keid; Trícia Maria F Sousa Oliveira; Rodrigo M Soares; Matheus C Martini; Maria A Orsi; Clarice W Arns; Helena L Ferreira

doi:10.1128/genomeA.01243-16

Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator)

Genome Announc. 2016 Nov 17;4(6):e01243-16. doi: 10.1128/genomeA.01243-16.

Authors

Steven Van Borm¹, Laís S Rizotto², Leila S Ullmann³, Guilherme P Scagion², Camila D Malossi³, Raphael M Simão², João P Araújo Jr³, Izabelle M Cordeiro², Lara B Keid^{4

2}, Trícia Maria F Sousa Oliveira^{4

2}, Rodrigo M Soares², Matheus C Martini⁵, Maria A Orsi⁶, Clarice W Arns⁵, Helena L Ferreira^{7

2}

Affiliations

¹ Molecular Platform Unit, CODA-CERVA Veterinary and Agrochemical Research Centre, Brussels, Belgium.
² Postgraduate Program in Experimental Epidemiology of Zoonoses, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), São Paulo, Brazil.
³ Laboratory of Animal and Human Virology, Department of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista, Botucatu, UNESP, São Paulo, Brazil.
⁴ Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA-USP), Pirassununga, Brazil.
⁵ Laboratory of Animal Virology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
⁶ National Agricultural Laboratory of Brazil, Lanagro/São Paulo, Campinas, Brazil.
⁷ Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA-USP), Pirassununga, Brazil [email protected].

Abstract

A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription-PCR (RRT-PCR) from a captive owl swab. The complete genome sequence of APMV-1/Rhinoptynx clamator/Brazil/22516/2009 (APMV-1, avian paramyxovirus type 1) was obtained using Illumina sequencing. Phylogenetic analysis of the complete genome classified the isolate within NDV class II genotype II.