Selective reduction of IFN-γ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection

Catherine Riou; Rubina Bunjun; Tracey L Müller; Agano Kiravu; Zekarias Ginbot; Tolu Oni; Rene Goliath; Robert J Wilkinson; Wendy A Burgers

doi:10.1016/j.tube.2016.07.018

Selective reduction of IFN-γ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection

Tuberculosis (Edinb). 2016 Dec:101:25-30. doi: 10.1016/j.tube.2016.07.018. Epub 2016 Aug 9.

Authors

Catherine Riou¹, Rubina Bunjun², Tracey L Müller², Agano Kiravu², Zekarias Ginbot², Tolu Oni³, Rene Goliath⁴, Robert J Wilkinson⁵, Wendy A Burgers⁶

Affiliations

¹ Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
² Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
³ Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Division of Public Health Medicine, School of Public Health and Family Medicine, University of Cape Town, South Africa.
⁴ Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
⁵ Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; The Francis Crick Institute Mill Hill Laboratory, London, United Kingdom; Department of Medicine, Imperial College London, United Kingdom.
⁶ Division of Medical Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa. Electronic address: [email protected].

Abstract

HIV-1 is recognized to increase the risk for tuberculosis even before CD4+ T cell deficiency is profound. To better understand how HIV-1 alters immunity to latent tuberculosis, we compared the magnitude and functional profile of mycobacteria-specific CD4+ T cells between HIV-uninfected and HIV-infected individuals, using flow cytometry. In HIV-1 infection, IFN-γ single positive mycobacteria-specific CD4+ T cells were decreased, while the frequency of polyfunctional cells (IFN-γ+IL-2+TNF-α+) remained unchanged. Moreover, the proportion of IFN-γ single positive cells correlated inversely with viral replication. Our results suggest that HIV-1 affects mycobacteria-specific cells differentially, depending on their functional capacity.

Keywords: BCG; ESAT-6/CFP-10; HIV; LTBI; Memory; Mycobacterium tuberculosis; PPD; Polyfunction.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

AIDS-Related Opportunistic Infections / complications
AIDS-Related Opportunistic Infections / immunology*
AIDS-Related Opportunistic Infections / virology
Adult
Blood Specimen Collection / methods
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes / immunology*
Female
Flow Cytometry
HIV-1 / isolation & purification*
Humans
Interferon-gamma / blood*
Latent Tuberculosis / complications
Latent Tuberculosis / immunology*
Male
Mycobacterium tuberculosis / immunology
Viral Load
Young Adult

Substances

Interferon-gamma

Abstract

Publication types

MeSH terms

Substances

Grants and funding