Abstract
Recombinant DNA technology has been central in answering some of the most important questions in immunology and has recently helped to define the complex of membrane associated proteins on T-cell surfaces responsible for antigen recognition. Here André Traunecker and colleagues describe attempts to facilitate the analysis of this complex using genetic engineering to produce solubilized T-cell receptor and associated molecules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Differentiation, T-Lymphocyte / isolation & purification
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Cloning, Molecular / methods*
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Genes, Synthetic
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Histocompatibility Antigens Class II / isolation & purification
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Humans
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Membrane Proteins / isolation & purification*
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Mice
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Protein Conformation
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / isolation & purification*
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Recombinant Fusion Proteins / isolation & purification*
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Recombinant Proteins / isolation & purification*
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Solubility
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T-Lymphocytes / analysis
Substances
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Antigens, Differentiation, T-Lymphocyte
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Histocompatibility Antigens Class II
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I-E-antigen
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Membrane Proteins
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Receptors, Antigen, T-Cell
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Recombinant Fusion Proteins
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Recombinant Proteins