Abstract
We reported the inhibition of α-Hemolysin (Hla) production in methicillin-resistant Staphylococcus aureus USA300 by osthole and further investigated the combination of osthole and baicalin in the treatment of staphylococcal pneumonia. Using cytotoxicity assays and a mouse model of intranasal lung infection, we evaluated the effect of combined therapy. Our results suggest that the combination of osthole and baicalin alleviated S. aureus-mediated A549 cell injury and protected mice from S. aureus pneumonia.
Keywords:
Alpha-hemolysin; Baicalin; Osthole; Staphylococcus aureus.
MeSH terms
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A549 Cells
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Animals
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Bacterial Toxins / antagonists & inhibitors*
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Coumarins / administration & dosage*
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Coumarins / pharmacology
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Disease Models, Animal
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Drug Therapy, Combination
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Flavonoids / administration & dosage*
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Flavonoids / pharmacology
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Gene Expression Regulation, Bacterial / drug effects
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Hemolysin Proteins / antagonists & inhibitors*
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Humans
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Methicillin-Resistant Staphylococcus aureus / enzymology
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Mice
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Microbial Sensitivity Tests
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Pneumonia, Staphylococcal / prevention & control*
Substances
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Bacterial Toxins
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Coumarins
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Flavonoids
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Hemolysin Proteins
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staphylococcal alpha-toxin
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baicalin
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osthol