Small Photoblinking Semiconductor Polymer Dots for Fluorescence Nanoscopy

Xuanze Chen; Rongqin Li; Zhihe Liu; Kai Sun; Zezhou Sun; Danni Chen; Gaixia Xu; Peng Xi; Changfeng Wu; Yujie Sun

doi:10.1002/adma.201604850

Small Photoblinking Semiconductor Polymer Dots for Fluorescence Nanoscopy

Adv Mater. 2017 Feb;29(5). doi: 10.1002/adma.201604850. Epub 2016 Nov 24.

Authors

Xuanze Chen¹, Rongqin Li¹, Zhihe Liu², Kai Sun², Zezhou Sun², Danni Chen³, Gaixia Xu³, Peng Xi¹, Changfeng Wu², Yujie Sun¹

Affiliations

¹ State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China.
² State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China.
³ Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China.

PMID: 27882627
DOI: 10.1002/adma.201604850

Abstract

Two types of small photoblinking Pdots with high brightness, strong photostability, and favorable biocompatibility, are designed. Super-resolution optical fluctuation imaging is achieved using these Pdots. Imaging of subcellular structures demonstrates that these small photoblinking Pdots are outstanding probes for fast, long-term super-resolution fluorescence imaging.

Keywords: SOFI; bioimaging; blinking-based statistical super-resolution imaging; photoblinking; semiconductor polymer dots.