Abstract
Osteoinductive characteristics of new osteoplastic materials based on demineralized bone matrix of xenogenic origin with high and controlled degree of purification were studied on the model of regeneration of critical-size cranial defects in rats using modern approaches, including histological analysis, evaluation of morphological parameters of the bone tissue obtained by micro-computed tomography, and estimation of bone tissue growth rate using in vivo fluorochrome label. Demineralized bone matrix and, to a much greater extent, its activated form containing modified recombinant growth factor rhBMP-2 with high content of the dimeric form exhibited osteoinductive activity.
Keywords:
BMP-2; bone tissue regeneration; defect of critical size; demineralized bone matrix; osteoinductive activity.
MeSH terms
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Animals
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Biocompatible Materials / pharmacology
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Bone Demineralization Technique / methods*
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Bone Morphogenetic Protein 2 / biosynthesis
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Bone Morphogenetic Protein 2 / genetics
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Bone Morphogenetic Protein 2 / pharmacology*
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Bone Regeneration / drug effects*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fluorescent Dyes
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Gene Expression
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Humans
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Immobilized Proteins / biosynthesis
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Immobilized Proteins / genetics
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Immobilized Proteins / pharmacology
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Male
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Osteogenesis / drug effects*
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Protein Multimerization
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Rats
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Rats, Wistar
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Skull / drug effects*
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Skull / injuries
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Skull / surgery
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Tissue Engineering
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Tissue Scaffolds*
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X-Ray Microtomography
Substances
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BMP2 protein, human
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Biocompatible Materials
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Bone Morphogenetic Protein 2
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Fluorescent Dyes
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Immobilized Proteins
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Recombinant Proteins