Genetic Environment of the blaKPC-2 Gene in a Klebsiella pneumoniae Isolate That May Have Been Imported to Russia from Southeast Asia

Antimicrob Agents Chemother. 2017 Jan 24;61(2):e01856-16. doi: 10.1128/AAC.01856-16. Print 2017 Feb.

Abstract

The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia, from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38-bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA-mrx-mphR), and a fragment of blaTEM with unique polymorphisms.

Keywords: IncFII; IncR; KPC-2; Klebsiella pneumoniae; transposons.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Asia, Southeastern
  • Drug Resistance, Bacterial / drug effects
  • Drug Resistance, Bacterial / genetics
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Macrolides / pharmacology
  • Microbial Sensitivity Tests
  • Operon
  • Plasmids / genetics*
  • Russia
  • Transposases / genetics
  • Vietnam
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Macrolides
  • Transposases
  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae