Differences in the Nasopharyngeal Microbiome During Acute Respiratory Tract Infection With Human Rhinovirus and Respiratory Syncytial Virus in Infancy

J Infect Dis. 2016 Dec 15;214(12):1924-1928. doi: 10.1093/infdis/jiw456.

Abstract

Respiratory viruses alter the nasopharyngeal microbiome and may be associated with a distinct microbial signature. To test this hypothesis, we compared the nasopharyngeal microbiome of 135 previously healthy infants with acute respiratory infection due to human rhinovirus (HRV; n = 52) or respiratory syncytial virus (RSV; n = 83). The nasopharyngeal microbiome was assessed by sequencing the V4 region of the 16S ribosomal RNA. Respiratory viruses were identified by quantitative reverse-transcription polymerase chain reaction. We found significant differences in the overall taxonomic composition and abundance of certain bacterial genera between infants infected with HRV and those infected with RSV. Our results suggest that respiratory tract viral infections are associated with different nasopharyngeal microbial profiles.

Keywords: children; human rhinovirus; microbiome; nasopharynx; respiratory syncytial virus.

Publication types

  • Comparative Study

MeSH terms

  • Bacteria / classification*
  • Bacteria / genetics*
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Female
  • Humans
  • Infant
  • Male
  • Microbiota*
  • Nasopharynx / microbiology*
  • Picornaviridae Infections / pathology*
  • Prospective Studies
  • RNA, Ribosomal, 16S / genetics
  • Respiratory Syncytial Virus Infections / complications*
  • Respiratory Tract Infections / pathology*
  • Sequence Analysis, DNA

Substances

  • DNA, Ribosomal
  • RNA, Ribosomal, 16S