RepB' is required in trans for the two single-strand DNA initiation signals in oriV of plasmid RSF1010

Y Honda; H Sakai; T Komano; M Bagdasarian

doi:10.1016/0378-1119(89)90261-8

RepB' is required in trans for the two single-strand DNA initiation signals in oriV of plasmid RSF1010

Gene. 1989 Aug 1;80(1):155-9. doi: 10.1016/0378-1119(89)90261-8.

Authors

Y Honda¹, H Sakai, T Komano, M Bagdasarian

Affiliation

¹ Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Japan.

PMID: 2792769
DOI: 10.1016/0378-1119(89)90261-8

Abstract

We have shown previously [Honda et al., Gene 68 (1988) 221-228] that the oriV region of the broad-host-range plasmid RSF1010 contained two single-strand DNA initiation signals (ssi) which have RSF1010-specific properties since they required one or more factors provided in trans by RSF1010 for their functional activities. We demonstrate here, by deletion analysis, that repB', one of the genes essential for the vegetative replication of RSF1010, produces a factor required for the function of ssi signals. It is conceivable that the RepB' protein and the two ssi signals, ssiA and ssiB, cooperatively compose plasmid-specific priming complexes which confer the broad-host-range property upon RSF1010.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Bacteriophages / genetics
DNA Replication*
DNA, Single-Stranded / genetics
Gene Expression Regulation, Viral
Genetic Vectors
Plasmids*
Restriction Mapping

Substances

Bacterial Proteins
DNA, Single-Stranded
RepB protein, Bacteria