Utility of the QuantiFERON-TB Gold In-Tube assay for the diagnosis of tuberculosis in Moroccan children

Int J Tuberc Lung Dis. 2016 Dec;20(12):1639-1646. doi: 10.5588/ijtld.16.0382.

Abstract

Setting: The utility of interferon-gamma release assays (IGRAs), such as the QuantiFERON-TB Gold In-Tube (QFT-GIT) test, in diagnosing active tuberculosis (TB) in children is unclear and depends on the epidemiological setting.

Objective: To evaluate the performance of QFT-GIT for TB diagnosis in children living in Morocco, an intermediate TB incidence country with high bacille Calmette-Gurin vaccination coverage.

Design: We prospectively recruited 109 Moroccan children hospitalised for clinically suspected TB, all of whom were tested using QFT-GIT.

Results: For 81 of the 109 children, the final diagnosis was TB. The remaining 28 children did not have TB. QFT-GIT had a sensitivity of 66% (95%CI 5277) for the diagnosis of TB, and a specificity of 100% (95%CI 88100). The tuberculin skin test (TST) had lower sensitivity, at 46% (95%CI 3360), and its concordance with QFT-GIT was limited (69%). Combining QFT-GIT and TST results increased sensitivity to 83% (95%CI 6992).

Conclusion: In epidemiological settings such as those found in Morocco, QFT-GIT is more sensitive than the TST for active TB diagnosis in children. Combining the TST and QFT-GIT would be useful for the diagnosis of active TB in children, in combination with clinical, radiological and laboratory data.

MeSH terms

  • Adolescent
  • BCG Vaccine / administration & dosage
  • Child
  • Child, Preschool
  • Humans
  • Incidence
  • Infant
  • Interferon-gamma Release Tests*
  • Morocco / epidemiology
  • Prospective Studies
  • Sensitivity and Specificity
  • Tuberculin Test*
  • Tuberculosis / diagnosis*
  • Tuberculosis / epidemiology*
  • Tuberculosis / prevention & control
  • Vaccination

Substances

  • BCG Vaccine