A mitochondria-targeting fluorescent probe for the selective detection of glutathione in living cells

Xue-Liang Liu; Li-Ya Niu; Yu-Zhe Chen; Mei-Ling Zheng; Yunxu Yang; Qing-Zheng Yang

doi:10.1039/c6ob02407f

A mitochondria-targeting fluorescent probe for the selective detection of glutathione in living cells

Org Biomol Chem. 2017 Feb 1;15(5):1072-1075. doi: 10.1039/c6ob02407f.

Authors

Xue-Liang Liu¹, Li-Ya Niu², Yu-Zhe Chen³, Mei-Ling Zheng³, Yunxu Yang¹, Qing-Zheng Yang²

Affiliations

¹ School of Chemistry and biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China. [email protected].
² Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. [email protected] [email protected].
³ Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

PMID: 28059419
DOI: 10.1039/c6ob02407f

Abstract

We report a fluorescent probe for the selective detection of mitochondrial glutathione (GSH). The probe, containing triphenylphosphine as a mitochondrial targeting group, exhibited ratiometric and selective detection of GSH over Cys/Hcy. The probe was used for imaging mitochondrial GSH in living HeLa cells.

MeSH terms

Fluorescent Dyes / analysis
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Glutathione / analysis*
HeLa Cells
Humans
Mitochondria / chemistry*
Molecular Structure

Substances

Fluorescent Dyes
Glutathione