Neurobrucellosis: Unexpected Answer From Metagenomic Next-Generation Sequencing

J Pediatric Infect Dis Soc. 2017 Nov 24;6(4):393-398. doi: 10.1093/jpids/piw066.

Abstract

A diagnosis of brucellosis can be difficult because routine culture and serological methods exhibit variable sensitivity and specificity. We present the use of a metagenomic next- generation sequencing assay to diagnose a case of neurobrucellosis from cerebrospinal fluid, resulting in the institution of appropriate antibiotic treatment and a favorable clinical outcome.

Keywords: diagnostic microbiology; encephalitis; meningitis; metagenomic next-generation sequencing; neurobrucellosis.

MeSH terms

  • Brucella / genetics
  • Brucellosis / cerebrospinal fluid
  • Brucellosis / diagnosis*
  • Child
  • Female
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Magnetic Resonance Imaging
  • Metagenomics / methods
  • Spinal Cord / diagnostic imaging