Abstract
We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.
MeSH terms
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DNA / analysis*
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DNA / chemical synthesis
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DNA / chemistry*
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Deoxyuridine / analogs & derivatives*
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Deoxyuridine / chemical synthesis
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Deoxyuridine / chemistry
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Oligonucleotides / analysis*
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Oligonucleotides / chemical synthesis*
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Oligonucleotides / chemistry
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Organophosphorus Compounds / chemical synthesis*
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Organophosphorus Compounds / chemistry
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Thymine / chemistry*
Substances
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Oligonucleotides
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Organophosphorus Compounds
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phosphoramidite
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5-formyl-2'-deoxyuridine
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DNA
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Thymine
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Deoxyuridine