Multimodality MRI assessment of grey and white matter injury and blood-brain barrier disruption after intracerebral haemorrhage in mice

Sci Rep. 2017 Jan 13:7:40358. doi: 10.1038/srep40358.

Abstract

In this study, we examined injury progression after intracerebral haemorrhage (ICH) induced by collagenase in mice using a preclinical 11.7 Tesla MRI system. On T2-weighted MRI, lesion and striatal volumes were increased on day 3 and then decreased from days 7 to 28. On day 3, with an increase in striatal water content, vasogenic oedema in the perihaematomal region presented as increased T2 and increased apparent diffusion coefficient (ADC) signal. With a synchronous change in T2 and ADC signals, microglial activation peaked on day 3 in the same region and decreased over time. Iron deposition appeared on day 3 around the haematoma border but did not change synchronously with ADC signals. Vascular permeability measured by Evans blue extravasation on days 1, 3, and 7 correlated with the T1-gadolinium results, both of which peaked on day 3. On diffusion tensor imaging, white matter injury was prominent in the corpus callosum and internal capsule on day 3 and then partially recovered over time. Our results indicate that the evolution of grey/white matter injury and blood-brain barrier disruption after ICH can be assessed with multimodal MRI, and that perihaematomal vasogenic oedema might be attributable to microglial activation, iron deposition, and blood-brain barrier breakdown.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood-Brain Barrier / diagnostic imaging*
  • Blood-Brain Barrier / physiopathology
  • Cerebral Hemorrhage / diagnostic imaging*
  • Cerebral Hemorrhage / physiopathology
  • Corpus Callosum / diagnostic imaging
  • Corpus Callosum / physiopathology
  • Diffusion Tensor Imaging
  • Disease Models, Animal
  • Gray Matter / diagnostic imaging*
  • Gray Matter / injuries
  • Gray Matter / physiopathology
  • Humans
  • Magnetic Resonance Imaging
  • Mice
  • Multimodal Imaging
  • White Matter / diagnostic imaging*
  • White Matter / injuries
  • White Matter / physiopathology