Associations between Rs4244285 and Rs762551 gene polymorphisms and age-related macular degeneration

Ophthalmic Genet. 2017 Jul-Aug;38(4):357-364. doi: 10.1080/13816810.2016.1242018. Epub 2017 Jan 17.

Abstract

Background: Age-related macular degeneration is the leading cause of blindness in elderly individuals in developed countries. The etiology and pathophysiology of age-related macular degeneration have not been elucidated yet. Knowing that the main pathological change of age-related macular degeneration is formation of drusen containing about 40% of lipids, there have been attempts to find associations between age-related macular degeneration and genes controlling lipid metabolism.

Purpose: To determine the frequency of CYP2C19 (G681A) Rs4244285 and CYP1A2 (-163C>A) Rs762551 genotypes in patients with age-related macular degeneration.

Methods: The study enrolled 150 patients with early age-related macular degeneration and 296 age- and gender-matched healthy controls. The genotyping of Rs4244285 and Rs762551 was carried out by using the real-time polymerase chain reaction method.

Results: The CYP1A2 (-163C>A) Rs762551 C/C genotype was more frequently detected in patients with age-related macular degeneration than in the control group (32.7% vs. 21.6%, p = 0.011) and was associated with an increased risk of developing early age-related macular degeneration (OR = 1.759, 95% CI: 1.133-2.729; p = 0.012). The CYP1A2 (-163C>A) Rs762551 C/A genotype was more frequently documented in the control group compared with patients with age-related macular degeneration (46.3% vs. 30.7%, p = 0.002) and was associated with a decreased risk of having age-related macular degeneration (OR = 0.580. 95% CI: 0.362-0.929, p = 0.023) in the co-dominant model.

Conclusion: The study showed that the CYP1A2 (-163C>A) Rs762551 C/C genotype was associated with an increased risk of age-related macular degeneration.

Keywords: Age-related macular degeneration; cytochrome P450; gene polymorphism.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Cytochrome P-450 CYP1A2 / genetics*
  • Cytochrome P-450 CYP2C19 / genetics*
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Macular Degeneration / diagnosis
  • Macular Degeneration / genetics*
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide*
  • Real-Time Polymerase Chain Reaction
  • Risk Factors

Substances

  • CYP1A2 protein, human
  • CYP2C19 protein, human
  • Cytochrome P-450 CYP1A2
  • Cytochrome P-450 CYP2C19