Abstract
Seven azaphilones, montagnuphilones A-G (1-7), together with previously known azaphilones 8-11, were encountered in Montagnulaceae sp. DM0194, an endophytic fungus isolated from submerged roots of Persicaria amphibia. The structures of 1-7 were elucidated on the basis of their MS and NMR spectroscopic analysis. Compounds 1-8 were evaluated for their cytotoxicity and ability to inhibit nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 macrophage cells. Among these, none were found to be cytotoxic to RAW264.7 cells up to 100.0 μM, but 8, 5, and 2 showed NO inhibitory activity with IC50 values of 9.2 ± 0.9, 25.5 ± 1.1, and 39.6 ± 1.8 μM, respectively.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, N.I.H., Extramural
MeSH terms
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Amphibians / microbiology*
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Animals
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Ascomycota / chemistry*
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Benzopyrans / chemistry*
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Benzopyrans / isolation & purification
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Endophytes / chemistry*
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Lipopolysaccharides / chemistry*
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Lipopolysaccharides / isolation & purification
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Lipopolysaccharides / pharmacology
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Macrophages / chemistry
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Macrophages / drug effects*
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Nitric Oxide / biosynthesis*
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Nitric Oxide / chemistry
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Pigments, Biological / chemistry*
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Pigments, Biological / isolation & purification
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Plant Roots / chemistry*
Substances
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Benzopyrans
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Lipopolysaccharides
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Pigments, Biological
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azaphilone
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Nitric Oxide