An oxygen sensitive self-decision making engineered CAR T-cell

Sci Rep. 2017 Jan 20:7:39833. doi: 10.1038/srep39833.

Abstract

A key to the success of chimeric antigen receptor (CAR) T-cell based therapies greatly rely on the capacity to identify and target antigens with expression restrained to tumor cells. Here we present a strategy to generate CAR T-cells that are only effective locally (tumor tissue), potentially also increasing the choice of targetable antigens. By fusing an oxygen sensitive subdomain of HIF1α to a CAR scaffold, we generated CAR T-cells that are responsive to a hypoxic environment, a hallmark of certain tumors. Along with the development of oxygen-sensitive CAR T-cells, this work also provides a basic framework to use a multi-chain CAR as a platform to create the next generation of smarter self-decision making CAR T-cells.

MeSH terms

  • Antigens, Neoplasm / immunology
  • Cell Line, Tumor
  • Cytotoxicity, Immunologic
  • Genetic Engineering
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
  • Immunotherapy, Adoptive / methods*
  • Lymphocyte Activation
  • Neoplasms / immunology
  • Neoplasms / therapy*
  • Oxygen / metabolism*
  • Receptors, Antigen, T-Cell / genetics*
  • Recombinant Fusion Proteins / genetics*
  • T-Cell Antigen Receptor Specificity
  • T-Lymphocytes, Cytotoxic / physiology*
  • T-Lymphocytes, Cytotoxic / transplantation
  • Tumor Microenvironment

Substances

  • Antigens, Neoplasm
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • Oxygen