Abstract
A high-quality custom database of MALDI-TOF mass spectral profiles was developed with the goal of improving clinical diagnostic identification of high-consequence bacterial pathogens. A biomarker discovery method is presented for identifying and evaluating MALDI-TOF MS spectra to potentially differentiate biothreat bacteria from less-pathogenic near-neighbour species.
Keywords:
Bioterrorism bacterial pathogens; Biothreat; Genus Yersinia, Bacillus, Brucella; MALDI-TOF mass spectrometry.
Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.
MeSH terms
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Bacillus / genetics
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Bacillus / isolation & purification
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Bacteria / genetics
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Bacteria / isolation & purification*
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Bacteria / pathogenicity*
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Bacterial Infections / diagnosis
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Bacterial Infections / microbiology
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Bacterial Proteins / isolation & purification
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Bacterial Typing Techniques*
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Biological Warfare Agents
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Biomarkers / analysis*
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Brucella / genetics
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Brucella / isolation & purification
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Databases, Nucleic Acid*
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Humans
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / standards
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Yersinia / genetics
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Yersinia / isolation & purification
Substances
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Bacterial Proteins
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Biological Warfare Agents
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Biomarkers