High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles

Nat Methods. 2017 Mar;14(3):283-286. doi: 10.1038/nmeth.4147. Epub 2017 Jan 23.

Abstract

Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.

MeSH terms

  • Escherichia coli / cytology*
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Nodaviridae / genetics
  • Nodaviridae / ultrastructure*
  • Protein Binding / physiology
  • RNA, Long Noncoding / metabolism*
  • Ribosome Subunits, Large, Bacterial / genetics
  • Ribosome Subunits, Large, Bacterial / ultrastructure*
  • Ribosome Subunits, Small, Bacterial / genetics
  • Ribosome Subunits, Small, Bacterial / ultrastructure*

Substances

  • RNA, Long Noncoding
  • steroid receptor RNA activator