Background: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis.
Methods: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016.
Results: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p<0.0001.
Conclusions: The IFA accurately expedites the diagnosis of melioidosis.
Keywords: Burkholderia pseudomallei; Bacteremia; Diagnosis; Immunofluorescent assay; Melioidosis; Thailand.
© The Author 2017. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.