Characterizing cell subsets using marker enrichment modeling

Nat Methods. 2017 Mar;14(3):275-278. doi: 10.1038/nmeth.4149. Epub 2017 Jan 30.

Abstract

Learning cell identity from high-content single-cell data presently relies on human experts. We present marker enrichment modeling (MEM), an algorithm that objectively describes cells by quantifying contextual feature enrichment and reporting a human- and machine-readable text label. MEM outperforms traditional metrics in describing immune and cancer cell subsets from fluorescence and mass cytometry. MEM provides a quantitative language to communicate characteristics of new and established cytotypes observed in complex tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms*
  • Biomarkers / analysis
  • Brain Neoplasms / immunology
  • Brain Neoplasms / pathology*
  • Computational Biology / methods*
  • Flow Cytometry / methods*
  • Glioblastoma / immunology
  • Glioblastoma / pathology*
  • Humans
  • Single-Cell Analysis / methods
  • T-Lymphocytes / cytology

Substances

  • Biomarkers