Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods

J Microbiol Methods. 2017 Apr:135:20-25. doi: 10.1016/j.mimet.2017.01.020. Epub 2017 Feb 1.

Abstract

The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.

Keywords: Danio rerio; Host-pathogen interaction; Immune response; Infection; Live-cell imaging; Salmonella; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified / immunology
  • Animals, Genetically Modified / microbiology
  • Disease Models, Animal
  • Host-Pathogen Interactions / immunology
  • Immersion
  • Immunity, Innate / immunology
  • Larva / immunology
  • Larva / microbiology*
  • Microinjections / methods*
  • Microinjections / veterinary*
  • Neutrophils / immunology
  • Salmonella Infections / diagnostic imaging*
  • Salmonella Infections / immunology
  • Salmonella Infections / microbiology*
  • Salmonella typhimurium / pathogenicity*
  • Zebrafish / immunology
  • Zebrafish / microbiology*