Virus-Mimetic Fusogenic Exosomes for Direct Delivery of Integral Membrane Proteins to Target Cell Membranes

Adv Mater. 2017 Apr;29(13). doi: 10.1002/adma.201605604. Epub 2017 Feb 6.

Abstract

An efficient system for direct delivery of integral membrane proteins is successfully developed using a new biocompatible exosome-based platform. Fusogenic exosomes harboring viral fusogen, vascular stomatitis virus (VSV)-G protein, can fuse with and modify plasma membranes in a process called "membrane editing." This can facilitate the transfer of biologically active membrane proteins into the target cell membranes both in vitro and in vivo.

Keywords: exosomes; membrane protein; nanoplatform; viral fusogen.

MeSH terms

  • Animals
  • Biological Therapy / methods
  • Biomimetic Materials* / metabolism
  • Cell Line
  • Cell Membrane / metabolism*
  • Endocytosis
  • Exosomes* / metabolism
  • Green Fluorescent Proteins / administration & dosage
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Hydrogen-Ion Concentration
  • Liposomes / metabolism
  • Membrane Proteins / administration & dosage*
  • Membrane Proteins / metabolism
  • Mice
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / diagnostic imaging
  • Muscle, Skeletal / metabolism
  • Polyethylene Glycols / metabolism
  • Viral Proteins* / metabolism
  • Viruses

Substances

  • Liposomes
  • Membrane Proteins
  • Viral Proteins
  • Green Fluorescent Proteins
  • Polyethylene Glycols