Endothelial dysfunction during long-term follow-up in children with STEC hemolytic-uremic syndrome

Pediatr Nephrol. 2017 Jun;32(6):1005-1011. doi: 10.1007/s00467-016-3574-3. Epub 2017 Feb 8.

Abstract

Background: Shiga-toxin-producing Escherichia coli (STEC)-associated hemolytic-uremic syndrome (HUS) is a major cause of acute kidney injury (AKI), especially in children. Its long-term outcome with respect to endothelial damage remains largely elusive.

Methods: This was a cross-sectional study in 26 children who had suffered from STEC-HUS in the past and achieved a complete recovery of renal function (eGFR >90 ml/min/1.73 m2). Skin microcirculation after local heating was assessed by laser Doppler fluximetry, carotid-femoral pulse wave velocity (PWV), carotid intima media thickness (cIMT), 24-h ambulatory blood pressure, and angiopoietin (Ang) 1 and 2 serum levels after a median follow-up period of 6.1 years. The results were compared to those of healthy controls.

Results: All patients were normotensive, mean eGFR was 102 (range 91-154) ml/min/1.73 m2, and 13 of the 26 patients showed albuminuria. Endothelial dysfunction was present in 13 patients, and the mean serum Ang2/Ang1 ratio was increased compared to healthy children (each p < 0.05). In contrast, mean values for PWV and cIMT in the patients did not differ from those of the controls. Endothelial dysfunction was significantly associated with younger age at STEC-HUS manifestation, time after HUS, and presence of albuminuria.

Conclusion: The results of this study highlight the need for long-term follow-up of STEC-HUS patients even after complete recovery of eGFR and lack of hypertension with respect to microvascular damage.

Keywords: Arterial stiffness; Endothelial dysfunction; Late-time renal sequelae; STEC hemolytic uremic syndrome; Skin microcirculation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury / blood
  • Acute Kidney Injury / etiology
  • Acute Kidney Injury / pathology
  • Adolescent
  • Age Factors
  • Albuminuria / blood
  • Angiopoietin-1 / blood
  • Angiopoietin-2 / blood
  • Blood Pressure Monitoring, Ambulatory
  • Carotid Intima-Media Thickness
  • Child
  • Child, Preschool
  • Cross-Sectional Studies
  • Endothelium, Vascular / pathology*
  • Escherichia coli Infections / blood
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / pathology*
  • Female
  • Follow-Up Studies
  • Glomerular Filtration Rate
  • Hemolytic-Uremic Syndrome / complications
  • Hemolytic-Uremic Syndrome / microbiology
  • Hemolytic-Uremic Syndrome / pathology*
  • Hemolytic-Uremic Syndrome / therapy
  • Humans
  • Hypertension / diagnosis
  • Infant
  • Male
  • Microvessels / pathology*
  • Pulse Wave Analysis
  • Renal Dialysis
  • Shiga-Toxigenic Escherichia coli / isolation & purification*
  • Skin / blood supply
  • Time Factors

Substances

  • ANGPT1 protein, human
  • ANGPT2 protein, human
  • Angiopoietin-1
  • Angiopoietin-2