Recruitment of PP1 to the centrosomal scaffold protein CEP192

Biochem Biophys Res Commun. 2017 Mar 18;484(4):864-870. doi: 10.1016/j.bbrc.2017.02.004. Epub 2017 Feb 8.

Abstract

Centrosomal protein of 192 kDa (CEP192) is a scaffolding protein that recruits the mitotic protein kinases Aurora A and PLK1 to the centrosome. Here we demonstrate that CEP192 also recruits the type one protein phosphatase (PP1) via a highly conserved KHVTF docking motif. The threonine of the KHVTF motif is phosphorylated during mitosis and protein kinase inhibition studies suggest this to be a PLK1-dependent process.

Keywords: CEP192; Centrosome; Mitosis; Polo-like kinase one; Protein phosphatase one.

MeSH terms

  • Binding Sites
  • Centrosome / metabolism*
  • Centrosome / ultrastructure
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Enzyme Activation
  • HeLa Cells
  • Humans
  • Mitosis / physiology*
  • Phosphorylation
  • Protein Binding
  • Receptors, Neuropeptide Y / chemistry*
  • Receptors, Neuropeptide Y / metabolism*

Substances

  • Cep192 protein, human
  • Chromosomal Proteins, Non-Histone
  • Receptors, Neuropeptide Y
  • neuropeptide Y4 receptor