Abstract
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
MeSH terms
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3T3 Cells
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Animals
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HEK293 Cells
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High-Throughput Nucleotide Sequencing / economics
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High-Throughput Nucleotide Sequencing / instrumentation
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High-Throughput Nucleotide Sequencing / methods*
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Humans
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Leukocytes, Mononuclear / physiology
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Macrophages / microbiology
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Macrophages / physiology
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Mice
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Mycobacterium tuberculosis / pathogenicity
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RNA, Messenger / genetics
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Sequence Analysis, RNA / economics
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Sequence Analysis, RNA / instrumentation
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Sequence Analysis, RNA / methods*
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Single-Cell Analysis / economics
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Single-Cell Analysis / instrumentation
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Single-Cell Analysis / methods*