Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America

Daniel Farias Monte; Andressa Mem; Miriam R Fernandes; Louise Cerdeira; Fernanda Esposito; Julia A Galvão; Bernadette D G M Franco; Nilton Lincopan; Mariza Landgraf

doi:10.1128/AAC.02718-16

Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America

Antimicrob Agents Chemother. 2017 Apr 24;61(5):e02718-16. doi: 10.1128/AAC.02718-16. Print 2017 May.

Authors

Daniel Farias Monte^{1

2}, Andressa Mem³, Miriam R Fernandes⁴, Louise Cerdeira⁴, Fernanda Esposito⁴, Julia A Galvão³, Bernadette D G M Franco^{1

2}, Nilton Lincopan^{5

6}, Mariza Landgraf^{1

2}

Affiliations

¹ Department of Food and Experimental Nutrition, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
² Food Research Center, University of São Paulo, São Paulo, Brazil.
³ Department of Veterinary Medicine, Federal University of Paraná, Paraná, Brazil.
⁴ Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
⁵ Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil [email protected].
⁶ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Abstract

The detection and rapid spread of colistin-resistant Enterobacteriaceae carrying the mcr-1 gene has created an urgent need to strengthen surveillance. In this study, eight clonally unrelated colistin-resistant Escherichia coli isolates carrying mcr-1 and bla_CTX-M or bla_CMY-2 genes were isolated from commercial chicken meat in Brazil. Most E. coli strains carried IncX4 plasmids, previously identified in human and animal isolates. These results highlight a new reservoir of mcr-1-harboring E. coli strains in South America.

Keywords: Brazil; CTX-M-8; ESBL; IncX4; mcr-1; polymyxins.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Brazil
Chickens / microbiology*
Colistin / pharmacology*
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / drug therapy
Escherichia coli Proteins / genetics*
Foodborne Diseases / drug therapy
Foodborne Diseases / microbiology
Foodborne Diseases / prevention & control
Humans
Meat Products / microbiology*
Plasmids / genetics
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-1 protein, E coli
beta-lactamase CMY-2
beta-Lactamases
beta-lactamase CTX-M, E coli
Colistin