Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles

Jicheng Yu; Yuqi Zhang; Zhen Gu

doi:10.1007/978-1-4939-6840-4_17

Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles

Methods Mol Biol. 2017:1570:251-259. doi: 10.1007/978-1-4939-6840-4_17.

Authors

Jicheng Yu^{1

2

3}, Yuqi Zhang^{1

2

3}, Zhen Gu^{4

5

6

7}

Affiliations

¹ Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
² North Carolina State University, 911 Oval Dr., Campus Mailbox 7115, Raleigh, NC, 27695, USA.
³ Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
⁴ Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. [email protected].
⁵ North Carolina State University, 911 Oval Dr., Campus Mailbox 7115, Raleigh, NC, 27695, USA. [email protected].
⁶ Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. [email protected].
⁷ Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. [email protected].

PMID: 28238142
DOI: 10.1007/978-1-4939-6840-4_17

Abstract

In this chapter, we describe the preparation of glucose-responsive vesicles (GRVs) and the fabrication of GRV-loaded microneedle-array patches for insulin delivery. The GRVs were formed of hypoxia-sensitive hyaluronic acid (HS-HA), the synthesis of which is presented in detail. We also describe the procedure to evaluate the in vivo efficacy of this smart patch in a mouse model of chemically induced type 1 diabetes through transcutaneous administration.

Keywords: Diabetes; Drug delivery; Glucose-responsive; Hypoxia-sensitive; Microneedle.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Administration, Cutaneous
Animals
Blood Glucose
Diabetes Mellitus, Experimental
Diabetes Mellitus, Type 1 / drug therapy
Drug Delivery Systems / methods*
Glucose / metabolism*
Hyaluronic Acid / chemical synthesis
Hyaluronic Acid / chemistry
Hypoxia*
Insulin / administration & dosage*
Male
Mice
Microinjections / methods*
Unilamellar Liposomes*

Substances

Blood Glucose
Insulin
Unilamellar Liposomes
Hyaluronic Acid
Glucose

Grants and funding

UL1 TR001111/TR/NCATS NIH HHS/United States