Multicenter evaluation of the revised RIDA® QUICK test (N1402) for rapid detection of norovirus in a diagnostic laboratory setting

Diagn Microbiol Infect Dis. 2017 May;88(1):31-35. doi: 10.1016/j.diagmicrobio.2017.02.006. Epub 2017 Feb 12.

Abstract

The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA® QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR®Green RT-rtPCR, but not by the RIDA® GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid). The RIDA® QUICK can be used to reliably confirm norovirus in stool samples, but a negative result does not definitively exclude the presence of norovirus.

Keywords: Antigen test; Norovirus; RIDA® gene; RIDA® quick; Xpert® norovirus.

Publication types

  • Evaluation Study
  • Multicenter Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Caliciviridae Infections / diagnosis*
  • Child
  • Chromatography, Affinity / methods*
  • Feces / virology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Norovirus / isolation & purification*
  • Prospective Studies
  • Sensitivity and Specificity
  • Time Factors
  • Young Adult