Technical approaches to induce selective cell death of pluripotent stem cells

Cell Mol Life Sci. 2017 Jul;74(14):2601-2611. doi: 10.1007/s00018-017-2486-0. Epub 2017 Feb 28.

Abstract

Despite the recent promising results of clinical trials using human pluripotent stem cell (hPSC)-based cell therapies for age-related macular degeneration (AMD), the risk of teratoma formation resulting from residual undifferentiated hPSCs remains a serious and critical hurdle for broader clinical implementation. To mitigate the tumorigenic risk of hPSC-based cell therapy, a variety of approaches have been examined to ablate the undifferentiated hPSCs based on the unique molecular properties of hPSCs. In the present review, we offer a brief overview of recent attempts at selective elimination of undifferentiated hPSCs to decrease the risk of teratoma formation in hPSC-based cell therapy.

Keywords: Apoptosis; Human pluripotent stem cells; Safe stem cell therapy; Selective cell death; Teratoma.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / drug effects
  • Genes, Transgenic, Suicide
  • Humans
  • MicroRNAs / metabolism
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / metabolism
  • Small Molecule Libraries / pharmacology
  • Stem Cell Transplantation / methods*

Substances

  • MicroRNAs
  • Small Molecule Libraries