Objective: To investigate the effects of miR-382 on cell migration, invasion and proliferation of gastric cancer cell lines MGC-803. Methods: The level of miR-382 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues. miR-382 overexpression was achieved by transfection of construct pcDNA-miR-382 into MGC-803 cells. The migration, invasion and proliferation of MGC-803 cells were detected by the scratch wound assay, Transwell and CCK-8, respectively. Results: miR-382 was decreased in 41 cases (82%) of gastric cancer tissues compared to their control. Furthermore, overexpression of miR-382 effectively inhibited the migration, invasion and proliferation of MGC-803 cells(P<0.05). Conclusion: Down-regulation of miR-382 has a correlation with the progression of gastric cancer. Up-regulation of miR-382 can inhibit the migration, invasion and proliferation of MGC-803 cells.
目的：探讨miR-382对胃癌细胞系MGC-803迁移、侵袭和增殖的影响。 方法：实时(Real-time)聚合酶链反应(PCR)分析50例胃癌组织(C)及其癌旁正常组织(N)中miR-382的表达水平；用pcDNA3.1载体构建过表达miR-382的重组质粒(pcDNA-miR-382)，实现miR-382在MGC-803细胞中的过表达；分别用划痕实验、Transwell和CCK-8法检测细胞迁移、侵袭和增殖能力。 结果：与癌旁对照组相比，41例(82%)胃癌组织中出现miR-382的表达下调；过表达miR-382能显著抑制MGC-803细胞的迁移、侵袭和增殖能力(P<0.05)。 结论： miR-382的表达下调可能与胃癌的进展有关，过表达miR-382可以抑制胃癌MGC-803细胞的迁移、侵袭和增殖。.
Keywords: Gastric cancer; Invasion; Migration; Proliferation; miR-382.