Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke; Alvaro J Benitez; Victoria Chalker; Karolina Gullsby; Birgit Henrich; Carlos Hidalgo-Grass; Theo Hoogenboezem; Darja Kese; Katherine Loens; Jolanda Maaskant; Ayelet Michael-Gayego; Allon E Moses; Ran Nir-Paz; Suzan D Pas; Sabine Pereyre; Randi F Petersen; Meike Rosenblatt; Annemarie M C van Rossum; Søren A Uldum; Wendy W J Unger; Dominique Ursi; Jonas M Winchell; Cecile Bebear; ESCMID Study Group for Mycoplasma Infections

doi:10.1016/j.diagmicrobio.2017.03.004

Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Diagn Microbiol Infect Dis. 2017 Jun;88(2):111-114. doi: 10.1016/j.diagmicrobio.2017.03.004. Epub 2017 Mar 8.

Authors

Roger Dumke¹, Alvaro J Benitez², Victoria Chalker³, Karolina Gullsby⁴, Birgit Henrich⁵, Carlos Hidalgo-Grass⁶, Theo Hoogenboezem⁷, Darja Kese⁸, Katherine Loens⁹, Jolanda Maaskant¹⁰, Ayelet Michael-Gayego¹¹, Allon E Moses¹¹, Ran Nir-Paz¹¹, Suzan D Pas¹⁰, Sabine Pereyre¹², Randi F Petersen¹³, Meike Rosenblatt⁵, Annemarie M C van Rossum¹⁴, Søren A Uldum¹³, Wendy W J Unger⁷, Dominique Ursi⁹, Jonas M Winchell², Cecile Bebear¹²; ESCMID Study Group for Mycoplasma Infections

Affiliations

¹ TU Dresden, Dresden, Germany. Electronic address: [email protected].
² Centers for Disease Control and Prevention, Atlanta, GA, USA.
³ Public Health England, London, United Kingdom.
⁴ Centre for Research and Development, Uppsala University/Region Gävleborg, Sweden.
⁵ Universität Düsseldorf, Düsseldorf, Germany.
⁶ HGC Biomedical Research Diagnostics S.L., Huelva, Spain.
⁷ Laboratory of Pediatrics, Department of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
⁸ University of Ljubljana, Ljubljana, Slovenia.
⁹ Universitair Ziekenhuis Antwerpen, Edegem, Belgium.
¹⁰ Viroscience, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
¹¹ Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
¹² University of Bordeaux, Bordeaux, France.
¹³ Statens Serum Institut, Copenhagen, Denmark.
¹⁴ Division of Infectious Diseases and Immunology, Department of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.

PMID: 28318608
DOI: 10.1016/j.diagmicrobio.2017.03.004

Abstract

Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.

Keywords: Detection; Multi-center evaluation; Mycoplasma pneumoniae; Real-time PCR.

Publication types

Multicenter Study

MeSH terms

DNA, Bacterial / analysis*
DNA, Bacterial / genetics
Genome, Bacterial / genetics*
Humans
Mycoplasma pneumoniae / genetics*
Mycoplasma pneumoniae / isolation & purification
Pneumonia, Mycoplasma / diagnosis*
Pneumonia, Mycoplasma / microbiology
Real-Time Polymerase Chain Reaction / methods*

Substances

DNA, Bacterial