IL-6, TNF-α, IL-10, and nutritional status in pediatric patients with biliary atresia

J Pediatr (Rio J). 2017 Sep-Oct;93(5):517-524. doi: 10.1016/j.jped.2016.11.009. Epub 2017 Mar 19.

Abstract

Objectives: The objective of the present study is to evaluate whether IL-6, TNF-α, IL-10 are associated with nutritional status in patients with cirrhosis secondary to biliary atresia and compare to healthy controls.

Methods: The parameters used for nutritional assessment were the standard deviation scores of height-for-age and of triceps skinfold thickness-for-age. The severity of cirrhosis was evaluated using the Child-Pugh score and PELD/MELD. Serum cytokines were measured using Cytometric Bead Array flow cytometry.

Results: IL-6, TNF-α, and IL-10 were significantly higher in the cirrhosis group when compared with the control group (2.4 vs. 0.24 (p<0.001), 0.21 vs. 0.14 (p=0.007), and 0.65 vs. 0.36 (p=0.004), respectively. IL-6 and IL-10 were positively correlated with disease severity (0.450 [p=0.001] and 0.410; [p=0.002], respectively). TNF-α did not show a significant correlation with disease severity (0.100; p=0.478). Regarding nutritional evaluation, IL-6 was negatively correlated with the standard deviation score of height-for-age (-0.493; p<0.001) and of triceps skinfold thickness-for-age (-0.503; p<0.001), respectively. IL-10 exhibited a negative correlation with the standard deviation score of height-for-age (-0.476; p<0.001) and the standard deviation score of triceps skinfold thickness-for-age (-0.388; p=0.004). TNF-α did not show any significance in both anthropometric parameters (-0.083 (p=0.555) and -0.161 (p=0.253).

Conclusion: The authors suggest that, in patients with cirrhosis secondary to biliary atresia, IL-6 could be used as a possible supporting biomarker of deficient nutritional status and elevated IL-10 levels could be used as a possible early-stage supporting biomarker of deteriorating nutritional status.

Keywords: Atresia Biliar; Biliary atresia; Cirrhosis; Cirrose; Estado nutricional; IL-10; IL-6; Nutritional status; TNF-α.

MeSH terms

  • Biliary Atresia / blood*
  • Biliary Atresia / complications
  • Biliary Atresia / immunology
  • Biomarkers / blood
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Interleukin-10 / blood*
  • Interleukin-10 / immunology
  • Interleukin-6 / blood*
  • Interleukin-6 / immunology
  • Liver Cirrhosis / blood*
  • Liver Cirrhosis / etiology
  • Liver Cirrhosis / immunology
  • Male
  • Nutrition Assessment
  • Nutritional Status*
  • Severity of Illness Index
  • Tumor Necrosis Factor-alpha / blood*
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Biomarkers
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10