Neuronal and Peripheral Pentraxins Modify Glutamate Release and may Interact in Blood-Brain Barrier Failure

Cereb Cortex. 2017 Jun 1;27(6):3437-3448. doi: 10.1093/cercor/bhx046.

Abstract

Neuronal pentraxin 1 (NPTX1) has been implicated in Alzheimer's disease, being present in and around dystrophic neurons in plaques, affecting glutamatergic transmission postsynaptically and mediating effects of amyloidβ. Here, we confirm the presence of NPTX1 around plaques in postmortem Alzheimer's disease brain and report that acutely applied human NPTX1 increases paired-pulse ratio at mouse CA3-CA1 hippocampal synapses, indicating a decrease in glutamate release. In contrast, chronic exposure to NPTX1, NPTX2, or NPTX receptor decreases paired-pulse ratio, mimicking some of the earliest changes in mice expressing familial Alzheimer's disease genes. The peripheral pentraxin, serum amyloid P component (SAP), causes similar synaptic effects to NPTX1. The presence of SAP on amyloid plaques in Alzheimer's disease confirms that it can enter the brain. We show that SAP and neuronal pentraxins can interact and that SAP can enter the brain if the blood-brain barrier is compromised, suggesting that peripheral pentraxins could affect central synaptic transmission via this interaction, especially in the event of blood-brain barrier breakdown.

Keywords: Alzheimer's disease; central nervous system; neurodegenerative disease; presynaptic; synaptic plasticity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged, 80 and over
  • Alzheimer Disease / pathology
  • Animals
  • Animals, Newborn
  • Blood-Brain Barrier / pathology
  • Blood-Brain Barrier / physiopathology*
  • C-Reactive Protein / genetics
  • C-Reactive Protein / metabolism*
  • C-Reactive Protein / pharmacology
  • Evoked Potentials / drug effects
  • Evoked Potentials / physiology
  • Female
  • GABA Antagonists / pharmacology
  • Glutamic Acid / metabolism*
  • HEK293 Cells
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Hippocampus / pathology
  • Hippocampus / physiology*
  • Humans
  • Lipopolysaccharides / pharmacology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Middle Aged
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nerve Tissue Proteins / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Pyridazines / pharmacology
  • Serum Amyloid P-Component / pharmacology
  • Synapses / drug effects
  • Synapses / genetics
  • Synapses / metabolism

Substances

  • GABA Antagonists
  • Lipopolysaccharides
  • Nerve Tissue Proteins
  • Pyridazines
  • Serum Amyloid P-Component
  • neuronal pentraxin
  • Glutamic Acid
  • C-Reactive Protein
  • gabazine