TLR2 knockout protects against diabetes-mediated changes in cerebral perfusion and cognitive deficits

Am J Physiol Regul Integr Comp Physiol. 2017 Jun 1;312(6):R927-R937. doi: 10.1152/ajpregu.00482.2016. Epub 2017 Feb 22.

Abstract

The risk of cognitive decline in diabetes (Type 1 and Type 2) is significantly greater compared with normoglycemic patients, and the risk of developing dementia in diabetic patients is doubled. The etiology for this is likely multifactorial, but one mechanism that has gained increasing attention is decreased cerebral perfusion as a result of cerebrovascular dysfunction. The innate immune system has been shown to play a role in diabetic vascular complications, notably through the Toll-like receptor (TLR)-stimulated release of proinflammatory cytokines and chemokines that lead to vascular damage. TLR2 has been implicated in playing a crucial role in the development of diabetic microvascular complications, such as nephropathy, and thus, we hypothesized that TLR2-mediated cerebrovascular dysfunction leads to decreased cerebral blood flow (CBF) and cognitive impairment in diabetes. Knockout of TLR2 conferred protection from impaired CBF in early-stage diabetes and from hyperperfusion in long-term diabetes, prevented the development of endothelium-dependent vascular dysfunction in diabetes, created a hyperactive and anxiolytic phenotype, and protected against diabetes-induced impairment of long-term hippocampal and prefrontal cortex-mediated fear learning. In conclusion, these findings support the involvement of TLR2 in the pathogenesis of diabetic vascular disease and cognitive impairment.

Keywords: Toll-like receptor 2; cerebral perfusion; cerebrovascular; cognitive impairment; diabetes.

MeSH terms

  • Animals
  • Aorta / drug effects
  • Aorta / physiopathology
  • Behavior, Animal*
  • Cerebrovascular Circulation*
  • Cognition Disorders / genetics
  • Cognition Disorders / metabolism
  • Cognition Disorders / prevention & control*
  • Cognition Disorders / psychology
  • Cognition*
  • Diabetes Mellitus, Experimental / complications*
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetic Angiopathies / genetics
  • Diabetic Angiopathies / metabolism
  • Diabetic Angiopathies / physiopathology
  • Diabetic Angiopathies / prevention & control*
  • Dose-Response Relationship, Drug
  • Exploratory Behavior
  • Fear
  • Genetic Predisposition to Disease
  • Hippocampus / metabolism
  • Hippocampus / physiopathology
  • Male
  • Maze Learning
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Motor Activity
  • Neuronal Plasticity
  • Phenotype
  • Prefrontal Cortex / metabolism
  • Prefrontal Cortex / physiopathology
  • Recognition, Psychology
  • Time Factors
  • Toll-Like Receptor 2 / deficiency*
  • Toll-Like Receptor 2 / genetics
  • Vasodilation / drug effects
  • Vasodilator Agents / pharmacology

Substances

  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Vasodilator Agents