FunResDB-A web resource for genotypic susceptibility testing of Aspergillus fumigatus

Med Mycol. 2018 Jan 1;56(1):117-120. doi: 10.1093/mmy/myx015.

Abstract

Therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in Aspergillus fumigatus. A majority of resistant strains carries mutations in the CYP51A gene. Due to a lack of sensitivity of culture-based methods, molecular detection of A. fumigatus has become an important diagnostic tool. We set up the database FunResDB (www.nrz-myk.de/funresdb) to gather all available information about CYP51A-dependent azole resistance from published literature. In summary, the screening resulted in 79 CYP51A variants, which are linked to 59 nonsynonymous mutations. A tailor-made online sequence analysis tool allows for genotypic susceptibility testing of A. fumigatus.

Keywords: Aspergillus fumigatus; database; drug susceptibility; fungal infection; triazoles.

MeSH terms

  • Alleles
  • Antifungal Agents / pharmacology*
  • Aspergillus fumigatus / drug effects*
  • Aspergillus fumigatus / genetics*
  • Azoles / pharmacology
  • Cytochrome P-450 Enzyme System / genetics
  • Databases, Genetic*
  • Fungal Proteins / genetics
  • Genotype
  • Genotyping Techniques / methods*
  • Humans
  • Internet*
  • Microbial Sensitivity Tests / methods*
  • Polymorphism, Genetic

Substances

  • Antifungal Agents
  • Azoles
  • Fungal Proteins
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450 CYP51A, Aspergillus