ADP-glucose pyrophosphorylase genes, associated with kernel weight, underwent selection during wheat domestication and breeding

Plant Biotechnol J. 2017 Dec;15(12):1533-1543. doi: 10.1111/pbi.12735. Epub 2017 May 12.

Abstract

ADP-glucose pyrophosphorylase, comprising two small subunits and two large subunits, is considered a key enzyme in the endosperm starch synthesis pathway in wheat (Triticum aestivum L.). Two genes, TaAGP-S1-7A and TaAGP-L-1B, were investigated in this study. Haplotypes of these genes were associated with thousand kernel weight (TKW) in different populations. Mean TKWs of favoured haplotypes were significantly higher than those of nonfavoured ones. Two molecular markers developed to distinguish these haplotypes could be used in molecular breeding. Frequencies of favoured haplotypes were dramatically increased in cultivars released in China after the 1940s. These favoured haplotypes were also positively selected in six major wheat production regions globally. Selection of AGP-S1 and AGP-L-1B in wheat mainly occurred during and after hexaploidization. Strong additive effects of the favoured haplotypes of with other genes for starch synthesis were also detected in different populations.

Keywords: Triticum aestivum; ADP-glucose pyrophosphorylase; association analysis; haplotype; selection; yield.

MeSH terms

  • China
  • Chromosome Mapping
  • Cloning, Molecular
  • Domestication
  • Genetic Markers
  • Glucose-1-Phosphate Adenylyltransferase / genetics*
  • Haplotypes
  • Plant Proteins / genetics*
  • Polymorphism, Single Nucleotide
  • Polyploidy
  • Promoter Regions, Genetic
  • Seeds / genetics
  • Seeds / physiology*
  • Selection, Genetic
  • Triticum / genetics*

Substances

  • Genetic Markers
  • Plant Proteins
  • Glucose-1-Phosphate Adenylyltransferase

Associated data

  • GENBANK/EF405961
  • GENBANK/AY727927
  • GENBANK/DQ839506