Differential expression of ST6GAL1 in the tumor progression of colorectal cancer

Sen Zhang; Jishun Lu; Zhijue Xu; Xia Zou; Xue Sun; Yingjiao Xu; Aidong Shan; Jiaoyang Lu; Xialin Yan; Yalu Cui; Wei Yan; Yuguo Du; Jianguo Gu; Minhua Zheng; Bo Feng; Yan Zhang

doi:10.1016/j.bbrc.2017.03.167

Differential expression of ST6GAL1 in the tumor progression of colorectal cancer

Biochem Biophys Res Commun. 2017 May 13;486(4):1090-1096. doi: 10.1016/j.bbrc.2017.03.167. Epub 2017 Apr 1.

Authors

Sen Zhang¹, Jishun Lu², Zhijue Xu³, Xia Zou⁴, Xue Sun⁵, Yingjiao Xu³, Aidong Shan³, Jiaoyang Lu¹, Xialin Yan¹, Yalu Cui³, Wei Yan³, Yuguo Du⁵, Jianguo Gu⁶, Minhua Zheng¹, Bo Feng⁷, Yan Zhang⁸

Affiliations

¹ Department of General Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin Er Road, Shanghai 200025, China.
² Key Laboratory of Systems Biomedicine (Ministry of Education), and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine (SCSB), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai 981-8558, Miyagi, Japan.
³ Key Laboratory of Systems Biomedicine (Ministry of Education), and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine (SCSB), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
⁴ Department of General Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin Er Road, Shanghai 200025, China; Key Laboratory of Systems Biomedicine (Ministry of Education), and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine (SCSB), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
⁵ State Key Lab of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
⁶ Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai 981-8558, Miyagi, Japan; Department of Pharmacology, Pharmacy College, Nantong University, Nantong 226001, Jiangsu, China.
⁷ Department of General Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin Er Road, Shanghai 200025, China. Electronic address: [email protected].
⁸ Key Laboratory of Systems Biomedicine (Ministry of Education), and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine (SCSB), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address: [email protected].

PMID: 28377225
DOI: 10.1016/j.bbrc.2017.03.167

Abstract

Elevated expression of β-galactoside α2,6-sialyltranferase 1 (ST6GAL1) has been observed in colorectal cancer (CRC) and demonstrated to be important for its tumorigenesis. Here, we found that ST6GAL1 expression was significantly higher in non-metastatic tumors (stage I and II) than that in metastatic tumors (stage III and IV) using 62 pair-matched tumor/normal tissues. To elucidate the molecular mechanisms of how ST6GAL1 affected the CRC progression, we performed a global identification of the substrates of ST6GAL1 in the colon adenocarcinoma cell line SW480. A total of 318 membrane proteins were identified differentially affected by ST6GAL1 overexpression using metabolic labeling and proteomic analysis. Subsequent bioinformatic analysis revealed a list of potential substrates that might mediate the different functions of ST6GAL1 in CRC including cell movement, cell death and survival. Taken together, these results indicate a dynamic change in the expression of ST6GAL1 during the CRC progression and provide a list of sialylated proteins potentially relevant to the different functions of ST6GAL1 in CRC.

Keywords: Colorectal cancer; Metabolic labeling; ST6GAL1; Sialylation.

MeSH terms

Antigens, CD / metabolism*
Cell Proliferation*
Colorectal Neoplasms / metabolism*
Colorectal Neoplasms / pathology*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic*
Humans
Neoplasm Invasiveness
Sialic Acids / metabolism*
Sialyltransferases / metabolism*
Tumor Cells, Cultured

Substances

Antigens, CD
Sialic Acids
Sialyltransferases
ST6GAL1 protein, human