Human placental calcitonin receptors

Biochem J. 1988 Mar 15;250(3):877-82. doi: 10.1042/bj2500877.

Abstract

Receptors for the hypocalcaemic hormone, calcitonin (CT), have been identified in a membrane fraction prepared from term human placentae. Binding of 125I-labelled salmon CT (125I-sCT) to the membranes was time- and temperature-dependent, saturable (Bmax. 58 +/- 11 fmol/mg of protein), of high affinity (Kd 80 +/- 21 pM) and poorly reversible. Species-specific CTs and CT analogues competed for 125I-sCT binding with potencies proportional to their known biological potencies. Various unrelated peptide hormones did not compete, indicating that receptor binding was specific for CT. Photoaffinity labelling using a derivatized biologically active sCT analogue, [Arg11,18,3-nitrophenylazide-Lys14]sCT, identified a receptor component of Mr approximately 85,000, comparable with findings in osteoclasts and other target cells. The presence of CT receptors in the human placenta supports other evidence that CT may have a role in the regulation of placental function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Buffers
  • Calcitonin / analogs & derivatives
  • Calcitonin / metabolism*
  • Cell Membrane / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Hydrogen-Ion Concentration
  • Membrane Proteins / metabolism
  • Placenta / metabolism*
  • Pregnancy
  • Receptors, Calcitonin
  • Receptors, Cell Surface / metabolism*

Substances

  • Buffers
  • Membrane Proteins
  • Receptors, Calcitonin
  • Receptors, Cell Surface
  • calcitonin, Arg-3-nitrophenylazido-Lys-
  • Calcitonin
  • Adenylyl Cyclases