Apparatus for Histological Validation of In Vivo and Ex Vivo Magnetic Resonance Imaging of the Human Prostate

Roger M Bourne; Colleen Bailey; Edward William Johnston; Hayley Pye; Susan Heavey; Hayley Whitaker; Bernard Siow; Alex Freeman; Greg L Shaw; Ashwin Sridhar; Thomy Mertzanidou; David J Hawkes; Daniel C Alexander; Shonit Punwani; Eleftheria Panagiotaki

doi:10.3389/fonc.2017.00047

Apparatus for Histological Validation of In Vivo and Ex Vivo Magnetic Resonance Imaging of the Human Prostate

Front Oncol. 2017 Mar 24:7:47. doi: 10.3389/fonc.2017.00047. eCollection 2017.

Authors

Affiliations

¹ Discipline of Medical Radiation Sciences, Faculty of Health Sciences, University of Sydney , Sydney, NSW , Australia.
² Centre for Medical Image Computing, University College London , London , UK.
³ Centre for Medical Imaging, University College London , London , UK.
⁴ Centre for Molecular Intervention, University College London , London , UK.
⁵ Centre for Advanced Biomedical Imaging, University College London , London , UK.
⁶ Department of Research Pathology, University College London , London , UK.
⁷ Division of Surgery and Interventional Science, University College London, London, UK; Department of Urology, University College London Hospitals, London, UK.

Abstract

This article describes apparatus to aid histological validation of magnetic resonance imaging studies of the human prostate. The apparatus includes a 3D-printed patient-specific mold that facilitates aligned in vivo and ex vivo imaging, in situ tissue fixation, and tissue sectioning with minimal organ deformation. The mold and a dedicated container include MRI-visible landmarks to enable consistent tissue positioning and minimize image registration complexity. The inclusion of high spatial resolution ex vivo imaging aids in registration of in vivo MRI and histopathology data.

Keywords: MRI validation; co-registration; ex vivo MRI; fixation; histology; in vivo MRI; prostate; prostate cancer.

Abstract

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