Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Stem Cell Res. 2017 Jan:18:26-28. doi: 10.1016/j.scr.2016.12.004. Epub 2016 Dec 10.

Abstract

Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.

MeSH terms

  • Animals
  • Antigens, CD34 / metabolism
  • Cell Line
  • Cellular Reprogramming*
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Induced Pluripotent Stem Cells / transplantation
  • Karyotype
  • Lentivirus / genetics
  • Male
  • Megakaryocyte Progenitor Cells / cytology*
  • Megakaryocyte Progenitor Cells / metabolism
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Platelet Membrane Glycoprotein IIb / metabolism
  • Teratoma / metabolism
  • Teratoma / pathology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Antigens, CD34
  • Platelet Membrane Glycoprotein IIb
  • Transcription Factors