Generation of human iPSCs from urine derived cells of a non-affected control subject

Stem Cell Res. 2017 Jan:18:33-36. doi: 10.1016/j.scr.2016.12.008. Epub 2016 Dec 9.

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Sequence
  • Cell Culture Techniques / methods
  • Cell Line
  • Cellular Reprogramming*
  • Embryoid Bodies / metabolism
  • Embryoid Bodies / pathology
  • Female
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Genotype
  • Heterozygote
  • Homozygote
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Microscopy, Fluorescence
  • Plasminogen Activator Inhibitor 1 / genetics
  • Plasminogen Activator Inhibitor 1 / metabolism
  • Sendai virus / genetics
  • Sequence Analysis, DNA
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Urine / cytology*

Substances

  • Plasminogen Activator Inhibitor 1
  • Transcription Factors