Arabidopsis leucine-rich repeat receptor-like kinase NILR1 is required for induction of innate immunity to parasitic nematodes

PLoS Pathog. 2017 Apr 13;13(4):e1006284. doi: 10.1371/journal.ppat.1006284. eCollection 2017 Apr.

Abstract

Plant-parasitic nematodes are destructive pests causing losses of billions of dollars annually. An effective plant defence against pathogens relies on the recognition of pathogen-associated molecular patterns (PAMPs) by surface-localised receptors leading to the activation of PAMP-triggered immunity (PTI). Extensive studies have been conducted to characterise the role of PTI in various models of plant-pathogen interactions. However, far less is known about the role of PTI in roots in general and in plant-nematode interactions in particular. Here we show that nematode-derived proteinaceous elicitor/s is/are capable of inducing PTI in Arabidopsis in a manner dependent on the common immune co-receptor BAK1. Consistent with the role played by BAK1, we identified a leucine-rich repeat receptor-like kinase, termed NILR1 that is specifically regulated upon infection by nematodes. We show that NILR1 is essential for PTI responses initiated by nematodes and nilr1 loss-of-function mutants are hypersusceptible to a broad category of nematodes. To our knowledge, NILR1 is the first example of an immune receptor that is involved in induction of basal immunity (PTI) in plants or in animals in response to nematodes. Manipulation of NILR1 will provide new options for nematode control in crop plants in future.

MeSH terms

  • Animals
  • Arabidopsis / cytology
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / immunology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Host-Parasite Interactions*
  • Plant Diseases / immunology*
  • Plant Immunity*
  • Plant Roots / cytology
  • Plant Roots / enzymology
  • Plant Roots / genetics
  • Plant Roots / immunology
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Signal Transduction
  • Tylenchoidea / physiology*

Substances

  • Arabidopsis Proteins
  • Protein Kinases
  • NILR1 protein, Arabidopsis
  • Protein Serine-Threonine Kinases

Grants and funding

This work was supported by a grant from German Research Foundation to SS (Grant SI 1739/5-1). The work in CZ lab is supported by the Gatsby Charitable Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.