Development of high efficacy peptide coated iron oxide nanoparticles encapsulated amphotericin B drug delivery system against visceral leishmaniasis

Rishikesh Kumar; Krishna Pandey; Ganesh C Sahoo; Susmita Das; Vnr Das; R K Topno; Pradeep Das

doi:10.1016/j.msec.2017.02.145

Development of high efficacy peptide coated iron oxide nanoparticles encapsulated amphotericin B drug delivery system against visceral leishmaniasis

Mater Sci Eng C Mater Biol Appl. 2017 Jun 1:75:1465-1471. doi: 10.1016/j.msec.2017.02.145. Epub 2017 Mar 4.

Authors

Rishikesh Kumar¹, Krishna Pandey², Ganesh C Sahoo³, Susmita Das⁴, Vnr Das⁵, R K Topno⁵, Pradeep Das⁴

Affiliations

¹ Biomedical and Nanomedicine Department, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Patna, India.
² Clinical Medicine Department, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Patna, India. Electronic address: [email protected].
³ Biomedical and Nanomedicine Department, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Patna, India. Electronic address: [email protected].
⁴ Microbiology Department, All India Institute of Medical Science (AIIMS), Patna, India.
⁵ Clinical Medicine Department, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Patna, India.

PMID: 28415438
DOI: 10.1016/j.msec.2017.02.145

Abstract

Visceral leishmaniasis, a protozoan disease transmitted by phlebotomine sandflies which affect mostly in Indian sub-continent. The treatment for visceral leishmaniasis (VL) caused by Leishmania donovani are limited and unsatisfactory. Currently available drug against such as miltefosine and polymer based drugs amBisome has high efficacy against VL but found serious side effects and poor absorbance. To overcome this, we developed peptide (glycine) coated iron oxide (Fe₃O₄₎ nanoparticles (GINPs) encapsulated amphoterecin B (AmB) drug against visceral leishmaniasis. Synthesis of GINPs was carried out and different characterization technique used to confirm the synthesis and size of GINPs GINPs-AmB showed that particle size in the range of 10-15nm and closed to spherical in shaped. GINPs-AmB showed release rate of AmB is higher at lower pH. Significantly two fold higher efficacies of GINP encapsulated AmB during in vitro study. There was a substantial reduction in the total parasite burden in spleen in treated groups (GINPs encapsulate AmB), compare to AmB alone. The results obtained from this study revealed that AmB loaded GINPs is twofold effective than AmB and therefore, it opens a new avenue for use of AmB loaded GINPs against VL.

Keywords: AmB; Drug delivery; Electron microscope; Leishmaniasis; Nanotechnology.

MeSH terms

Amphotericin B* / chemistry
Amphotericin B* / pharmacokinetics
Amphotericin B* / pharmacology
Animals
Coated Materials, Biocompatible* / chemistry
Coated Materials, Biocompatible* / pharmacokinetics
Coated Materials, Biocompatible* / pharmacology
Ferric Compounds* / chemistry
Ferric Compounds* / pharmacokinetics
Ferric Compounds* / pharmacology
Humans
Leishmania donovani*
Leishmaniasis, Visceral / drug therapy*
Magnetite Nanoparticles / chemistry*
Mice
Mice, Inbred BALB C
Peptides* / chemistry
Peptides* / pharmacology

Substances

Coated Materials, Biocompatible
Ferric Compounds
Magnetite Nanoparticles
Peptides
ferric oxide
Amphotericin B